منابع مشابه
De Novo Sequencing of Nonribosomal Peptides
While nonribosomal peptides (NRPs) are of tremendous pharmacological importance, there is currently no technology capable of highthroughput sequencing of NRPs. Difficulties in sequencing NRPs slow down the progress in elucidating the non-ribosomal genetic code and negatively affect various screening programs aimed at the discovery of natural compounds of medical importance. We propose to employ...
متن کاملDe novo sequencing of peptides by MS/MS.
The current status of de novo sequencing of peptides by MS/MS is reviewed with focus on collision cell MS/MS spectra. The relation between peptide structure and observed fragment ion series is discussed and the exhaustive extraction of sequence information from CID spectra of protonated peptide ions is described. The partial redundancy of the extracted sequence information and a high mass accur...
متن کاملConstrained De Novo Sequencing of Peptides with Application to Conotoxins
We describe algorithms for incorporating prior sequence knowledge into the candidate generation stage of de novo peptide sequencing by tandem mass spectrometry. We focus on two types of prior knowledge: homology to known sequences encoded by a regular expression or position-specific score matrix, and amino acid content encoded by a multiset of required residues. We show an application to de nov...
متن کاملDe novo peptide sequencing by deep learning.
De novo peptide sequencing from tandem MS data is the key technology in proteomics for the characterization of proteins, especially for new sequences, such as mAbs. In this study, we propose a deep neural network model, DeepNovo, for de novo peptide sequencing. DeepNovo architecture combines recent advances in convolutional neural networks and recurrent neural networks to learn features of tand...
متن کاملDe novo sequencing of peptides on single resin beads by MALDI-FTICR tandem mass spectrometry.
An efficient approach in combinatorial chemistry is the synthesis of one-bead-one-compound peptide libraries. In contrast to synthesis and functional screening, which is performed in a largely automated manner, structure determination has been frequently laborious and time-consuming. Here we report an approach for de novo sequencing of peptides on single beads by matrix-assisted laser desorptio...
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ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2009
ISSN: 1615-9853
DOI: 10.1002/pmic.200900459